Publications | ballestrem-lab

2022

Tensin3 interaction with talin drives the formation of fibronectin-associated fibrillar adhesions

Paul Atherton*, Rafaella Konstantinou*, Suat Peng Neo, Emily Wang, Eleonora Balloi, Marina Ptushkina, Hayley Bennett, Kath Clark, Jayantha Gunaratne, David Critchley, Igor Barsukov, Edward Manser, Christoph Ballestrem

J Cell Biol (2022) 221 (10): e202107022.

2021

Desmosome dualism – most of the junction is stable, but a plakophilin moiety is persistently dynamic
Judith B. Fülle, Henri Huppert, David Liebl, Jaron Liu, Rogerio Alves de Almeida,

Bian Yanes, Graham D. Wright, E. Birgitte Lane, David R. Garrod, Christoph Ballestrem

Journal of Cell Science (2021) 134 (21): jcs258906

Vinculin is required for neuronal mechanosensing but not for axon outgrowth

De-Yao Wang, Cristina Melero, Ashwaq Albaraky, Paul Atherton, Karin A. Jansen, Andrea Dimitracopoulos, Federico Dajas-Bailador, Adam Reid, Kristian Franze, Christoph Ballestrem

Experimental Cell Research. 2021 Oct 15;407(2):112805

2021

2020

An End to End System for Measuring Axon Growth

Z Liu, T Cootes, C Ballestrem

International Workshop on Machine Learning in Medical Imaging, 455-464

Estimation of Cell Cycle States of Human Melanoma Cells with Quantitative Phase Imaging and Deep Learning

Tristan Henser-Brownhill, Robert J Ju, Nikolas K Haass, Samantha J Stehbens, Christoph Ballestrem, Timothy F Cootes

2020 IEEE 17th International Symposium on Biomedical Imaging (ISBI), 1617-1621

Relief of talin autoinhibition triggers a force-independent association with vinculin

Paul Atherton, Franziska Lausecker, Alexandre Carisey, Andrew Gilmore, David Critchley, Igor Barsukov, Christoph Ballestrem

The Journal of Cell Biology 219 (1)

2019

Vinculins interaction with talin is essential for mammary epithelial differentiation

Pengbo Wang, Jian Wu, Amber Wood, Matthew Jones, Robert Pedley, Weiping Li, Robert S Ross, Christoph Ballestrem, Andrew P Gilmore, Charles H Streuli

Scientific reports 9 (1), 1-11

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Cristina Melero, Aljona Kolmogorova, Paul Atherton, Brian Derby, Adam Reid, Karin Jansen, Christoph Ballestrem

JoVE (Journal of Visualized Experiments), e60092

GAS2-like 1 coordinates cell division through its association with end-binding proteins

A Nazgiewicz, P Atherton, C Ballestrem

Scientific reports 9 (1), 1-10

Force-independent interactions of talin and vinculin govern integrin-mediated mechanotransduction

Paul Atherton, Franziska Lausecker, Alexandre Carisey, Andrew Gilmore, David Critchley, Igor Barsukov, Christoph Ballestrem

bioRxiv, 629683

2019

2018

Vinculin is required to maintain glomerular barrier integrity

Franziska Lausecker, Xuefei Tian, Kazunori Inoue, Zhen Wang, Christopher E Pedigo, Hossam Hassan, Chang Liu, Margaret Zimmer, Stephanie Jinno, Abby L Huckle, Hellyeh Hamidi, Robert S Ross, Roy Zent, Christoph Ballestrem, Rachel Lennon, Shuta Ishibe

Kidney international 93 (3), 643-655

Photoresponsive hydrogels with photoswitchable mechanical properties allow time-resolved analysis of cellular responses to matrix stiffening

I-Ning Lee, Oana Dobre, David Richards, Christoph Ballestrem, Judith M Curran, John A Hunt, Stephen M Richardson, Joe Swift, Lu Shin Wong

ACS applied materials & interfaces 10 (9), 7765-7776

2017

Mechanotransduction at the cell-matrix interface

KA Jansen, P Atherton, C Ballestrem

Seminars in cell & developmental biology 71, 75-83

Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity

P Atherton, F Lausecker, A Harrison, C Ballestrem

Journal of Cell Science 130 (14), 2277-2291

Distinct focal adhesion protein modules control different aspects of mechanotransduction

B Stutchbury, P Atherton, R Tsang, DY Wang, C Ballestrem

Journal of cell science 130 (9), 1612-1624

Talin gets SHANKed in the fight for integrin activation

P Atherton, C Ballestrem

Nature cell biology 19 (4), 265-267

Mechanobiology and the Kidney Glomerulus

F Lausecker, C Ballestrem, R Lennon

Mechanobiology: Exploitation for Medical Benefit, 161-173

2016

LD motif recognition by talin: structure of the talin-DLC1 complex

Thomas Zacharchenko, Xiaolan Qian, Benjamin T Goult, Devina Jethwa, Teresa B Almeida, Christoph Ballestrem, David R Critchley, Douglas R Lowy, Igor L Barsukov

Structure 24 (7), 1130-1141

Mechanosensitive components of integrin adhesions: Role of vinculin

Paul Atherton, Ben Stutchbury, Devina Jethwa, Christoph Ballestrem

Experimental cell research 343 (1), 21-27

Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition

Edward R Horton, Jonathan D Humphries, Ben Stutchbury, Guillaume Jacquemet, Christoph Ballestrem, Simon T Barry, Martin J Humphries

Journal of Cell Biology 212 (3), 349-364

2015

Vinculin controls talin engagement with the actomyosin machinery

Paul Atherton, Ben Stutchbury, De-Yao Wang, Devina Jethwa, Ricky Tsang, Eugenia Meiler-Rodriguez, Pengbo Wang, Neil Bate, Roy Zent, Igor L Barsukov, Benjamin T Goult, David R Critchley, Christoph Ballestrem

Nature communications 6 (1), 1-12

< 2016

2014

Combining AFM and acoustic probes to reveal changes in the elastic stiffness tensor of living cells

N Nijenhuis, X Zhao, A Carisey, C Ballestrem, B Derby

Biophysical journal 107 (7), 1502-1512

GAS2-like proteins mediate communication between microtubules and actin through interactions with end-binding proteins

Matthew J Stroud, Alicja Nazgiewicz, Edward A McKenzie, Yisu Wang, Richard A Kammerer, Christoph Ballestrem

Journal of cell science 127 (12), 2672-2682

2013

RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover

Benjamin T Goult, Thomas Zacharchenko, Neil Bate, Ricky Tsang, Fiona Hey, Alexandre R Gingras, Paul R Elliott, Gordon CK Roberts, Christoph Ballestrem, David R Critchley, Igor L Barsukov

Journal of Biological Chemistry 288 (12), 8238-8249

Syndecan-4 phosphorylation is a control point for integrin recycling

Mark R Morgan, Hellyeh Hamidi, Mark D Bass, Stacey Warwood, Christoph Ballestrem, Martin J Humphries

Developmental cell 24 (5), 472-485

Vinculin regulates the recruitment and release of core focal adhesion proteins in a force-dependent manner

Alex Carisey, Ricky Tsang, Alexandra M Greiner, Nadja Nijenhuis, Nikki Heath, Alicja Nazgiewicz, Ralf Kemkemer, Brian Derby, Joachim Spatz, Christoph Ballestrem

Current biology 23 (4), 271-281

Frequency Domain-Fluorescence Lifetime Imaging Microscopy (FD-FLIM) to investigate conformational changes of integrins.

E Balloi, B Atkinson, C Ballestrem

MOLECULAR BIOLOGY OF THE CELL 24

2012

β1 integrin NPXY motifs regulate kidney collecting-duct development and maintenance by induced-fit interactions with cytosolic proteins

Sijo Mathew, Zhenwei Lu, Riya J Palamuttam, Glenda Mernaugh, Arina Hadziselimovic, Jiang Chen, Nada Bulus, Leslie S Gewin, Markus Voehler, Alexander Meves, Christoph Ballestrem, Reinhard Fässler, Ambra Pozzi, Charles R Sanders, Roy Zent

Molecular and cellular biology 32 (20), 4080-4091

Spectraplakins promote microtubule-mediated axonal growth by functioning as structural microtubule-associated proteins and EB1-dependent+ TIPs (tip interacting proteins)

Juliana Alves-Silva, Natalia Sánchez-Soriano, Robin Beaven, Melanie Klein, Jill Parkin, Thomas H Millard, Hugo J Bellen, Koen JT Venken, Christoph Ballestrem, Richard A Kammerer, Andreas Prokop

Journal of Neuroscience 32 (27), 9143-9158

Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA

NO Deakin, C Ballestrem, CE Turner

PloS one 7 (5), e37990

Multi-layer phase analysis: quantifying the elastic properties of soft tissues and live cells with ultra-high-frequency scanning acoustic microscopy

Xuegen Zhao, Riaz Akhtar, Nadja Nijenhuis, Steven J Wilkinson, Lilli Murphy, Christoph Ballestrem, Michael J Sherratt, Rachel EB Watson, Brian Derby

IEEE transactions on ultrasonics, ferroelectrics, and frequency control 59 (4), 610-620

2011

The C terminus of talin links integrins to cell cycle progression

P Wang, C Ballestrem, CH Streuli

The Journal of cell biology 195 (3), 499

Characterization of G2L3 (GAS2-like 3), a new microtubule-and actin-binding protein related to spectraplakins

MJ Stroud, RA Kammerer, C Ballestrem

Journal of Biological Chemistry 286 (28), 24987-24995

Vinculin, an adapter protein in control of cell adhesion signalling

A Carisey, C Ballestrem

European journal of cell biology 90 (2-3), 157-163

Fluorescence recovery after photobleaching

A Carisey, M Stroud, R Tsang, C Ballestrem

Cell Migration, 387-402

2010

Kinectin-mediated endoplasmic reticulum dynamics supports focal adhesion growth in the cellular lamella

Xin Zhang, Yee Han Tee, Justin K Heng, Yajuan Zhu, Xian Hu, Felix Margadant, Christoph Ballestrem, Alexander Bershadsky, Gareth Griffiths, Hanry YuJournal of cell science 123 (22), 3901-3912

The kinetics of force‐induced cell reorganization depend on microtubules and actin

AM Goldyn, P Kaiser, JP Spatz, C Ballestrem, R Kemkemer

Cytoskeleton 67 (4), 241-250

The Rac activator STEF (Tiam2) regulates cell migration by microtubule‐mediated focal adhesion disassembly

Claire Rooney, Gavin White, Alicja Nazgiewicz, Simon A Woodcock, Kurt I Anderson, Christoph Ballestrem, Angeliki Malliri

EMBO reports 11 (4), 292-298

Focal adhesions are sites of integrin extension

Janet A Askari, Christopher J Tynan, Stephen ED Webb, Marisa L Martin-Fernandez, Christoph Ballestrem, Martin J Humphries

Journal of Cell Biology 188 (6), 891-903

Drosophila growth cones: A genetically tractable platform for the analysis of axonal growth dynamics

Natalia Sánchez‐Soriano, Catarina Gonçalves‐Pimentel, Robin Beaven, Ulrike Haessler, Lisa Ofner‐Ziegenfuss, Christoph Ballestrem, Andreas Prokop

Developmental Neurobiology 70 (1), 58-71

2009

Vinculin controls talin engagement with the actomyosin machinery

Nature communications 6 (1), 1-12

Force-induced cell polarisation is linked to RhoA-driven microtubule-independent focal-adhesion sliding

AM Goldyn, BA Rioja, JP Spatz, C Ballestrem, R Kemkemer

Journal of cell science 122 (20), 3644-3651

Regulation of microtubule dynamics by inhibition of the tubulin deacetylase HDAC6

Yuliya Zilberman, Christoph Ballestrem, Letizia Carramusa, Ralph Mazitschek, Saadi Khochbin, Alexander Bershadsky

Journal of cell science 122 (19), 3531-3541

An integrin-α4–14-3-3ζ–paxillin ternary complex mediates localised Cdc42 activity and accelerates cell migration

Nicholas O Deakin, Mark D Bass, Stacey Warwood, Julia Schoelermann, Zohreh Mostafavi-Pour, David Knight, Christoph Ballestrem, Martin J Humphries

Journal of cell science 122 (10), 1654-1664

2008

How do cells decide which way to turn?: IL4A-5

M Bass, M Morgan, K Roach, Z Mostafavi-Pour, T Schoen, T Muramatsu, U Mayer, C Ballestrem, J Spatz, M Humphries

The Febs Journal 275

2007

Vinculin controls focal adhesion formation by direct interactions with talin and actin

JD Humphries, P Wang, C Streuli, B Geiger, MJ Humphries, C Ballestrem

The Journal of cell biology 179 (5), 1043-1057

Mammalian diaphanous-related formin Dia1 controls the organization of E-cadherin-mediated cell-cell junctions

L Carramusa, C Ballestrem, Y Zilberman, AD Bershadsky

Journal of cell science 120 (21), 3870-3882

Syndecan-4–dependent Rac1 regulation determines directional migration in response to the extracellular matrix

Mark D Bass, Kirsty A Roach, Mark R Morgan, Zohreh Mostafavi-Pour, Tobias Schoen, Takashi Muramatsu, Ulrike Mayer, Christoph Ballestrem, Joachim P Spatz, Martin J Humphries

Journal of Cell Biology 177 (3), 527-538

2006

Assembly and mechanosensory function of focal adhesions: experiments and models

Alexander D Bershadsky, Christoph Ballestrem, Letizia Carramusa, Yuliya Zilberman, Benoit Gilquin, Saadi Khochbin, Antonina Y Alexandrova, Alexander B Verkhovsky, Tom Shemesh, Michael M Kozlov

European journal of cell biology 85 (3-4), 165-173

Molecular mapping of tyrosine-phosphorylated proteins in focal adhesions using fluorescence resonance energy transfer

C Ballestrem, N Erez, J Kirchner, Z Kam, A Bershadsky, B Geiger

Journal of cell science 119 (5), 866-875

2005

Measurement of protein tyrosine phosphorylation in cell adhesion

VV Iyer, C Ballestrem, J Kirchner, B Geiger, MD Schaller

Cell Migration, 289-302

Application of microscope-based FRET to study molecular interactions in focal adhesions of live cells

C Ballestrem, B Geiger

Cell Migration, 321-334

2004

Y Zilberman, C Ballestrem, B Gilquin, S Khochbin, AD Bershadsky

MOLECULAR BIOLOGY OF THE CELL 15, 156A-157A

Interplay between the actin cytoskeleton, focal adhesions, and microtubules

C Ballestrem, N Magid, J Zonis, M Shtutman, A Bershadsky

Cell Motility: From Molecules to Cell Motility: From Molecules to Organisms. New York, NY: John Wiley & Sons, 75-99

Mechanosensory Function of Focal Adhesions

C Ballestrem, N Schiefermeier, J Zonis, K Arnold, AD Bershadsky

European Cells and Materials 7 (1), 3

Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion

Dirk Lehnert, Bernhard Wehrle-Haller, Christian David, Ulrich Weiland, Christoph Ballestrem, Beat A Imhof, Martin Bastmeyer

Journal of cell science 117 (1), 41-52

2003

Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells

R Zaidel-Bar, C Ballestrem, Z Kam, B Geiger

Journal of cell science 116 (22), 4605-4613

α-Smooth muscle actin is crucial for focal adhesion maturation in myofibroblasts

B Hinz, V Dugina, C Ballestrem, B Wehrle-Haller, C Chaponnier

Molecular biology of the cell 14 (6), 2508-2519

2002

Fluorescence resonance energy transfer (FRET) illuminates molecular interactions in focal adhesions of live cells

C Ballestrem, J Kirchner, Z Kam, A Bershadsky, B Geiger

MOLECULAR BIOLOGY OF THE CELL 13, 148A-148A

Three dimensional engagement of integrins affects morpholgy, migration and mechanical behavior of fibroblasts

KA Beningo, M Dembo, C Ballestrem, B Wehrle-Haller, Y Wang

MOLECULAR BIOLOGY OF THE CELL 13, 423A-423A

Regulation of microtubule dynamics by the formin homology protein, mDia1

C Ballestrem, N Magid, J Zonis, M Shtutman, T Ishizaki, S Narumiya, A Bershadsky

Molecular biology of the cell 13, 422A-422A

2001

JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells

M Aurrand-Lions, L Duncan, C Ballestrem, BA Imhof

Journal of Biological Chemistry 276 (4), 2733-2741

Differential αVβ3 integrin turnover regulates focal adhesion behavior and migration in mouse B16 melanoma cells

B Wehrle-Haller, C Ballestrem, B Hinz, B Imhof

Pigment Cell Research 14 (5), 399-400

Marching at the front and dragging behind: differential αVβ3-integrin turnover regulates focal adhesion behavior

C Ballestrem, B Hinz, BA Imhof, B Wehrle-Haller

The Journal of cell biology 155 (7), 1319-1332

2000

Actin-dependent lamellipodia formation and microtubule-dependent tail retraction control-directed cell migration

C Ballestrem, B Wehrle-Haller, B Hinz, BA Imhof

Molecular biology of the cell 11 (9), 2999-3012

Integration of atomic force and confocal microscopy

M Horton, G Charras, C Ballestrem, P Lehenkari

Single Molecules 1 (2), 135-137

PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin αvβ3 in cis

Cindy WY Wong, Guido Wiedle, Christoph Ballestrem, Bernhard Wehrle-Haller, Susanne Etteldorf, Monika Bruckner, Britta Engelhardt, Roland H Gisler, Beat A Imhof

Molecular biology of the cell 11 (9), 3109-3121

1999

Ultrasonics, Ferroelectrics and Frequency Control

J Vig, Y Kim

IEEE Transactions on 46, 1558

Endocytic vesicles move at the tips of actin tails in cultured mast cells

Christien J Merrifield, Stephen E Moss, Christoph Ballestrem, Beat A Imhof, Günter Giese, Ilse Wunderlich, Wolfhard Almers

Nature cell biology 1 (1), 72-74

1998

Actin dynamics in living mammalian cells

C Ballestrem, B Wehrle-Haller, BA Imhof

Journal of cell science 111 (12), 1649-1658

1997

An epitope on VLA-6 (α6β1) integrin involved in migration but not adhesion is required for extravasation of murine melanoma B16F1 cells in liver

D Hangan, VL Morris, L Boeters, C von Ballestrem, S Uniyal, BMC Chan

Cancer research 57 (17), 3812-3817

1996

Differential utilization of VLA‐4 (α4β1) and‐5 (α5β1) integrins during the development of mouse bone marrow‐derived mast cells

CC Fehlner‐Gardiner, S Uniyal, CG von Ballestrem, BMC Chan

Differentiation 60 (5), 317-325

Integrin VLA-2 (α2β1) function in postextravasation movement of human rhabdomyosarcoma RD cells in the liver

Dolores Hangan, Shashi Uniyal, Vincent L Morris, Ian C MacDonald, Christoph von Ballestrem, Thu Chau, Eric E Schmidt, Ann F Chambers, Alan C Groom, Bosco MC Chan

Cancer research 56 (13), 3142-3149

VLA-β1 Integrin Subunit-Specific Monoclonal Antibodies MB1.1 and MB1.2: Binding to Epitopes Not Dependent on Thymocyte Development or Regulated by Phorbol Ester and Divalent Cations

CHRISTOPH G. von BALLESTREM, SHASHI UNIYAL, JOHN I. McCORMICK, THU CHAU, BHAGIRATH SINGH, and BOSCO M.C. CHAN

Hybridoma 15 (2), 125-132